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Absorbance detector based on a deep UV light emitting diode for narrow-column HPLC

Bui, Duy Anh; Hauser, Peter C.; Bomastyk, Benjamin

http://repository.vnu.edu.vn/handle/VNU_123/28355

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  • Nhan đề:
    Absorbance detector based on a deep UV light emitting diode for narrow-column HPLC
  • Tác giả: Bui, Duy Anh; Hauser, Peter C.; Bomastyk, Benjamin
  • Chủ đề: Light-emitting diode; UV detection; Narrow-bore chromatography
  • Mô tả: Journal of Separation Science 36(19), pp. 3152-3157
    A detector for miniaturized HPLC based on deep UV emitting diodes and UV photodiodes was constructed. The measurement is accomplished by the transverse passage of the ra-diation from the light-emitting diode (LED) through fused-silica tubing with an internal diameter of 250 m. The optical cell allows flexible alignment of the LED, tubing, and pho-todiode for optimization of the light throughput and has an aperture to block stray light. A beam splitter was employed to direct part of the emitted light to a reference photodiode and the Lambert–Beer law was emulated with a log-ratio amplifier circuitry. The detector was testedwith two LEDs with emission bands at 280 and 255 nmand showed noise levels as low as 0.25 and 0.22 mAU, respectively. The photometric device was employed successfully in separations using a column of 1 mm inner diameter in isocratic as well as gradient elution. Good linearities over three orders of magnitude in concentration were achieved, and the precision of the measurements was better than 1% in all cases. Detection down to the low micromolar range was possible
  • Nơi xuất bản: H. : ĐHQGHN
  • Năm xuất bản: 2013
  • Ngôn ngữ: other
  • Số nhận dạng: http://repository.vnu.edu.vn/handle/VNU_123/28355

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