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WAT1 (WALLS ARE THIN1) defines a novel auxin transporter in plants and integrates auxin signaling in secondary wall formation in Arabidopsis fibers

Ranocha, Philippe ; Dima, Oana ; Felten, Judith ; Freydier, Amandine ; Hoffman, Laurent ; Ljung, Karin ; Lacombe, Benoit ; Corratgé, Claire ; Thibaud, Jean - Baptiste ; Sundberg, Björn ; Boerjan, Wout ; Goffner, Deborah

BMC PROCEEDINGS, 2011 [Tạp chí có phản biện]

ISSN: 1753-6561 ; DOI: 10.1186/1753-6561-5-S7-O24

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  • Nhan đề:
    WAT1 (WALLS ARE THIN1) defines a novel auxin transporter in plants and integrates auxin signaling in secondary wall formation in Arabidopsis fibers
  • Tác giả: Ranocha, Philippe ; Dima, Oana ; Felten, Judith ; Freydier, Amandine ; Hoffman, Laurent ; Ljung, Karin ; Lacombe, Benoit ; Corratgé, Claire ; Thibaud, Jean - Baptiste ; Sundberg, Björn ; Boerjan, Wout ; Goffner, Deborah
  • Chủ đề: Biology And Life Sciences
  • Là 1 phần của: BMC PROCEEDINGS, 2011
  • Mô tả: To authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1365-313X.2010.04256.x Keywords: Arabidopsis; secondary cell wall; fiber development; NODULIN21-like; tonoplast; plant drug/metabolite exporter Abstract: Summary By combining Zinnia elegans in vitro tracheary element genomics with reverse genetics in Arabidopsis, we have identified a new upstream component of secondary wall formation in xylary and interfascicular fibers. Walls are thin 1 (WAT1), an Arabidopsis thaliana homolog of Medicago truncatula NODULIN 21 (MtN21), encodes a plant-specific, predicted integral membrane protein, and is a member of the plant drug/metabolite exporter (P-DME) family (transporter classification number: TC 2.A.7.3). Although WAT1 is ubiquitously expressed throughout the plant, its expression is preferentially associated with vascular tissues, including developing xylem vessels and fibers. WAT1:GFP fusion protein analysis demonstrated that WAT1 is localized to the tonoplast. Analysis of wat1 mutants revealed two cell wall-related phenotypes in stems: a defect in cell elongation, resulting in a dwarfed habit and little to no secondary cell walls in fibers. Secondary walls of vessel elements were unaffected by the mutation. The secondary wall phenotype was supported by comparative transcriptomic and metabolomic analyses of wat1 and wild-type stems, as many transcripts and metabolites involved in secondary wall formation were reduced in abundance. Unexpectedly, these experiments also revealed a modification in tryptophan (Trp) and auxin metabolism that might contribute to the wat1 phenotype. Together, our data demonstrate an essential role for the WAT1 tonoplast protein in the control of secondary cell wall formation in fibers. Author Affiliation: (1)CNRS, Universite de Toulouse, UPS, UMR 5546, Surfaces Cellulaires et Signalisation chez les Vegetaux; BP 42617, F-31326, Castanet-Tolosan, France (2)VIB Department of Plant Systems Biology, UGent Department of Plant Biotechnology and Genetics, 9052 Gent, Belgium (3)Institut fuer Biologie III, Albert Ludwigs Universitaet Freiburg, 79104 Freiburg, Germany (4)Unite de Recherche en Genomique Vegetale INRA-CNRS, 91057 Evry, France (5)Umea Plant Science Center, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90183 Umea, Sweden Article History: Received 24 February 2010; revised 21 April 2010; accepted 5 May 2010. Article note: (*) For correspondence (fax +33 0 562193502; e-mail goffner@scsv.ups-tlse.fr).
  • Năm xuất bản: 2011
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 1753-6561 ; DOI: 10.1186/1753-6561-5-S7-O24

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