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PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTO

Edgar Antonio Reyes Montaño ; Leonardo Lareo ; Gerardo Pérez

Revista Colombiana de Química, 01 December 2011, Vol.41(1), pp.31-45 [Tạp chí có phản biện]

ISSN: 0120-2804 ; E-ISSN: 2357-3791

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  • Nhan đề:
    PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTO
  • Tác giả: Edgar Antonio Reyes Montaño ; Leonardo Lareo ; Gerardo Pérez
  • Chủ đề: Chemistry
  • Là 1 phần của: Revista Colombiana de Química, 01 December 2011, Vol.41(1), pp.31-45
  • Mô tả: Producing polyclonal antibodies (IgY) inchickens has advantages over those obtainedin other animal models, since theyhave been used as a tool for studyingdifferent proteins (NMDA glutamate receptorin our case, specifically the NR1subunit). We produced specific antibodiesagainst expression products by thealternative splicing of the gene encodingNMDA receptor NR1 subunit in adult ratbrain. Three peptides corresponding tothe splicing sites (N1, C1 and C2’ cassettes)were designed, synthesised and usedindividually as antigens in hens. Specificimmunoglobulins were purified fromyolks. The antibodies were then used forpurifying the NMDA receptor NR1 subunitusing affinity chromatography couplingthe three antibodies to the support.R
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 0120-2804 ; E-ISSN: 2357-3791

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