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Molecular bases of cyclodextrin adapter interactions with engineered protein nanopores

Banerjee, A. ; Mikhailova, E. ; Cheley, S. ; Gu, L.-Q. ; Montoya, M. ; Nagaoka, Y. ; Gouaux, E. ; Bayley, H.

Proceedings of the National Academy of Sciences, 05/04/2010, Vol.107(18), pp.8165-8170 [Tạp chí có phản biện]

ISSN: 0027-8424 ; E-ISSN: 1091-6490 ; DOI: http://dx.doi.org/10.1073/pnas.0914229107

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  • Nhan đề:
    Molecular bases of cyclodextrin adapter interactions with engineered protein nanopores
  • Tác giả: Banerjee, A. ; Mikhailova, E. ; Cheley, S. ; Gu, L.-Q. ; Montoya, M. ; Nagaoka, Y. ; Gouaux, E. ; Bayley, H.
  • Chủ đề: Cyclodextrins -- Properties ; Amino Acids -- Properties ; Dna Sequencing -- Methods ; Stochastic Processes -- Methods
  • Là 1 phần của: Proceedings of the National Academy of Sciences, 05/04/2010, Vol.107(18), pp.8165-8170
  • Mô tả: Engineered protein pores have several potential applications in biotechnology: as sensor elements in stochastic detection and ultrarapid DNA sequencing, as nanoreactors to observe single-molecule chemistry, and in the construction of nano-and microdevices. One important class of pores contains molecular adapters, which provide internal binding sites for small molecules. Mutants of the [alpha]-hemolysin ([alpha]HL) pore that bind the adapter [beta]-cyclodextrin ([beta]CD) ~[10.sup.4] times more tightly than the wild type have been obtained. We now use single-channel electrical recording, protein engineering including unnatural amino acid mutagenesis, and high-resolution x-ray crystallography to provide definitive structural information on these engineered protein nanopores in unparalleled detail. alpha-hemolysin | single molecute | stochastic sensing | structure | unnatural amino acid doi/ 10.1073/pnas.0914229107
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 0027-8424 ; E-ISSN: 1091-6490 ; DOI: http://dx.doi.org/10.1073/pnas.0914229107

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