skip to main content
Ngôn ngữ:
Giới hạn tìm kiếm: Giới hạn tìm kiếm: Dạng tài nguyên Hiển thị kết quả với: Hiển thị kết quả với: Chỉ mục

Temporal Changes in Cell Marker Expression and Cellular Infiltration in a Controlled Cortical Impact Model in Adult Male C57BL/6 Mice (Immune Cells after Traumatic Brain Injury)

Jin, Xuemei ; Ishii, Hiroshi ; Bai, Zhongbin ; Itokazu, Takahide ; Yamashita, Toshihide; Nataf, Serge (Editor)

PLoS ONE, 2012, Vol.7(7), p.e41892 [Tạp chí có phản biện]

E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0041892

Toàn văn sẵn có

Phiên bản sẵn có
Trích dẫn Trích dẫn bởi
  • Nhan đề:
    Temporal Changes in Cell Marker Expression and Cellular Infiltration in a Controlled Cortical Impact Model in Adult Male C57BL/6 Mice (Immune Cells after Traumatic Brain Injury)
  • Tác giả: Jin, Xuemei ; Ishii, Hiroshi ; Bai, Zhongbin ; Itokazu, Takahide ; Yamashita, Toshihide
  • Nataf, Serge (Editor)
  • Chủ đề: Research Article ; Biology ; Medicine ; Immunology ; Surgery ; Cell Biology ; Neuroscience ; Neurological Disorders
  • Là 1 phần của: PLoS ONE, 2012, Vol.7(7), p.e41892
  • Mô tả: Traumatic injury to the central nervous system (CNS) triggers a robust inflammatory response that leads to axonal damage and secondary degeneration of spared tissue. In contrast, some immune responses have neuroprotective effects. However, detailed information regarding the dynamics of immune responses after traumatic CNS injury is still unavailable. ; In the present study, changes in the immune cells present in the injured brain, spleen, and cervical lymph nodes (CLNs), which are draining lymphatic organs from the CNS, were analyzed after controlled cortical impact (CCI) by flow cytometry and immunohistochemistry. ; The number of neutrophils and macrophages that infiltrated the injured brain immediately increased 1 d post-injury and declined rapidly thereafter. In the injured brain, resident microglia showed a bimodal increase during the first week and in the chronic phase (≥3 weeks) after injury. Increase in the Iba-1 microglia/macrophages was observed around the injured site. Morphologic analysis showed that Iba-1 cells were round at 1 week, whereas those at 3 weeks were more ramified. Furthermore, CD86/CD11b M1-like microglia increased at 4 weeks after CCI, whereas CD206/CD11b M2-like microglia increased at 1 week. These results suggest that different subsets of microglia increased in the acute and chronic phases after CCI. Dendritic cells and T cells increased transiently within 1 week in the injured brain. In the CLNs and the spleen, T cells showed dynamic changes after CCI. In particular, the alteration in the number of T cells in the CLNs showed a similar pattern, with a 1-week delay, to that of microglia in the injured brain. ; The data from this study provide useful information on the dynamics of immune cells in CNS injuries.
  • Ngôn ngữ: English
  • Số nhận dạng: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0041892

Đang tìm Cơ sở dữ liệu bên ngoài...