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Genomic Confirmation of Hybridisation and Recent Inbreeding in a Vector-Isolated Leishmania Population (Sexual Reproduction in Leishmania)

Rogers, Matthew B ; Downing, Tim ; Smith, Barbara A ; Imamura, Hideo ; Sanders, Mandy ; Svobodova, Milena ; Volf, Petr ; Berriman, Matthew ; Cotton, James A ; Smith, Deborah F; Didelot, Xavier (Editor)

2014, Vol.10(1), p.e1004092 [Tạp chí có phản biện]

ISSN: 1553-7390 ; E-ISSN: 1553-7404 ; DOI: 10.1371/journal.pgen.1004092

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  • Nhan đề:
    Genomic Confirmation of Hybridisation and Recent Inbreeding in a Vector-Isolated Leishmania Population (Sexual Reproduction in Leishmania)
  • Tác giả: Rogers, Matthew B ; Downing, Tim ; Smith, Barbara A ; Imamura, Hideo ; Sanders, Mandy ; Svobodova, Milena ; Volf, Petr ; Berriman, Matthew ; Cotton, James A ; Smith, Deborah F
  • Didelot, Xavier (Editor)
  • Chủ đề: Research Article ; Biology ; Medicine ; Veterinary Science
  • Là 1 phần của: 2014, Vol.10(1), p.e1004092
  • Mô tả: Although asexual reproduction via clonal propagation has been proposed as the principal reproductive mechanism across parasitic protozoa of the Leishmania genus, sexual recombination has long been suspected, based on hybrid marker profiles detected in field isolates from different geographical locations. The recent experimental demonstration of a sexual cycle in Leishmania within sand flies has confirmed the occurrence of hybridisation, but knowledge of the parasite life cycle in the wild still remains limited. Here, we use whole genome sequencing to investigate the frequency of sexual reproduction in Leishmania , by sequencing the genomes of 11 Leishmania infantum isolates from sand flies and 1 patient isolate in a focus of cutaneous leishmaniasis in the Çukurova province of southeast Turkey. This is the first genome-wide examination of a vector-isolated population of Leishmania parasites. A genome-wide pattern of patchy heterozygosity and SNP density was observed both within individual strains and across the whole group. Comparisons with other Leishmania donovani complex genome sequences suggest that these isolates are derived from a single cross of two diverse strains with subsequent recombination within the population. This interpretation is supported by a statistical model of the genomic variability for each strain compared to the L. infantum reference genome strain as well as genome-wide scans for recombination within the population. Further analysis of these heterozygous blocks indicates that the two parents were phylogenetically distinct. Patterns of linkage disequilibrium indicate that this population reproduced primarily clonally following the original hybridisation event, but that some recombination also occurred. This observation allowed us to estimate the relative rates of sexual and asexual reproduction within this population, to our knowledge the first quantitative estimate of these events during the Leishmania life cycle. ; Sexual reproduction is predicted to be a rare event in parasites, as evidenced by detection of rare parasite hybrids in natural populations using molecular methods. Recently, a sexual cycle has been detected experimentally in parasites within the sand fly vector (that transmits this pathogenic microorganism to mammalian species including man, causing human leishmaniasis). In this study, we have used whole genome sequencing to investigate genetic variation at the highest level of resolution in parasites isolated from sand flies in a defined focus of leishmaniasis in southeast Turkey. Using a range of analytical tools, we show that variation in these parasites arose following a single cross between two diverse strains and subsequent recombination between the progeny, despite mainly clonal reproduction in the parasite population. We have thus been able to derive quantitative estimates of the relative rates of sexual and asexual reproduction during the life cycle for the first time, information that will be critical to our understanding of the epidemiology and evolution of this genus.
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 1553-7390 ; E-ISSN: 1553-7404 ; DOI: 10.1371/journal.pgen.1004092

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