skip to main content
Ngôn ngữ:
Giới hạn tìm kiếm: Giới hạn tìm kiếm: Dạng tài nguyên Hiển thị kết quả với: Hiển thị kết quả với: Chỉ mục

Codon usage limitation in the expression of HIV-1 envelope glycoprotein

Haas, J ; Park, E C ; Seed, B

Current biology : CB, 01 March 1996, Vol.6(3), pp.315-24 [Tạp chí có phản biện]

ISSN: 0960-9822 ; PMID: 8805248 Version:1

Toàn văn sẵn có

Phiên bản sẵn có
Trích dẫn Trích dẫn bởi
  • Nhan đề:
    Codon usage limitation in the expression of HIV-1 envelope glycoprotein
  • Tác giả: Haas, J ; Park, E C ; Seed, B
  • Chủ đề: Codon ; Gene Expression ; HIV Envelope Protein Gp120 -- Genetics ; HIV-1 -- Genetics
  • Là 1 phần của: Current biology : CB, 01 March 1996, Vol.6(3), pp.315-24
  • Mô tả: The expression of both the env and gag gene products of human immunodeficiency virus type 1 (HIV-1) is known to be limited by cis elements in the viral RNA that impede egress from the nucleus and reduce the efficiency of translation. Identifying these elements has proven difficult, as they appear to be disseminated throughout the viral genome. Here, we report that selective codon usage appears to account for a substantial fraction of the inefficiency of viral protein synthesis, independent of any effect on improved nuclear export. The codon usage effect is not specific to transcripts of HIV-1 origin. Re-engineering the coding sequence of a model protein (Thy-1) with the most prevalent HIV-1 codons significantly impairs Thy-1 expression, whereas altering the coding sequence of the jellyfish green fluorescent protein gene to conform to the favored codons of highly expressed human proteins results in a substantial increase in expression efficiency. Codon-usage effects are a major impediment to the efficient expression of HIV-1 genes. Although mammalian genes do not show as profound a bias as do Escherichia coli genes, other proteins that are poorly expressed in mammalian cells can benefit from codon re-engineering.
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 0960-9822 ; PMID: 8805248 Version:1

Đang tìm Cơ sở dữ liệu bên ngoài...