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Multidisciplinary evaluation of Clostridium butyricum clonality isolated from preterm neonates with necrotizing enterocolitis in South France between 2009 and 2017

Hosny, Michel ; Bou Khalil, Jacques Yaacoub ; Caputo, Aurelia ; Abdallah, Rita Abou ; Levasseur, Anthony ; Colson, Philippe ; Cassir, Nadim ; La Scola, Bernard

Scientific reports, 14 February 2019, Vol.9(1), pp.2077 [Tạp chí có phản biện]

E-ISSN: 2045-2322 ; PMID: 30765857 Version:1 ; DOI: 10.1038/s41598-019-38773-7

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  • Nhan đề:
    Multidisciplinary evaluation of Clostridium butyricum clonality isolated from preterm neonates with necrotizing enterocolitis in South France between 2009 and 2017
  • Tác giả: Hosny, Michel ; Bou Khalil, Jacques Yaacoub ; Caputo, Aurelia ; Abdallah, Rita Abou ; Levasseur, Anthony ; Colson, Philippe ; Cassir, Nadim ; La Scola, Bernard
  • Là 1 phần của: Scientific reports, 14 February 2019, Vol.9(1), pp.2077
  • Mô tả: The association between Clostridium species identification from stool samples in preterm neonates and the occurrence of necrotizing enterocolitis has been increasingly reported. To confirm the specific impact of Clostridium butyricum in this pathology, selective culture procedure was used for Clostridia isolation. Whole-genome analysis was employed to investigate genomic relationships between isolates. Stool samples from present study, as well as from previously investigated cases, were implicated including 88 from preterm neonates with necrotizing enterocolitis and 71 from matched controls. Quantitative real-time polymerase chain reaction was performed to evaluate the presence of C. butyricum from stools of new cases. Clostridium species prevalence isolated by culture was compared between patients with necrotizing enterocolitis and controls. By combining results of both culture and quantitative polymerase chain reaction methods, C. butyricum was significantly more frequent in stool samples...
  • Ngôn ngữ: English
  • Số nhận dạng: E-ISSN: 2045-2322 ; PMID: 30765857 Version:1 ; DOI: 10.1038/s41598-019-38773-7

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