skip to main content
Ngôn ngữ:
Giới hạn tìm kiếm: Giới hạn tìm kiếm: Dạng tài nguyên Hiển thị kết quả với: Hiển thị kết quả với: Chỉ mục

Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite Onchocerca volvulus and Its Wolbachia Endosymbiont

Bennuru, Sasisekhar ; Cotton, James A ; Ribeiro, Jose M C ; Grote, Alexandra ; Harsha, Bhavana ; Holroyd, Nancy ; Mhashilkar, Amruta ; Molina, Douglas M ; Randall, Arlo Z ; Shandling, Adam D ; Unnasch, Thomas R ; Ghedin, Elodie ; Berriman, Matthew ; Lustigman, Sara ; Nutman, Thomas B

mBio, 23 November 2016, Vol.7(6) [Tạp chí có phản biện]

E-ISSN: 2150-7511 ; PMID: 27881553 Version:1 ; DOI: 10.1128/mBio.02028-16

Toàn văn sẵn có

Trích dẫn Trích dẫn bởi
  • Nhan đề:
    Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite Onchocerca volvulus and Its Wolbachia Endosymbiont
  • Tác giả: Bennuru, Sasisekhar ; Cotton, James A ; Ribeiro, Jose M C ; Grote, Alexandra ; Harsha, Bhavana ; Holroyd, Nancy ; Mhashilkar, Amruta ; Molina, Douglas M ; Randall, Arlo Z ; Shandling, Adam D ; Unnasch, Thomas R ; Ghedin, Elodie ; Berriman, Matthew ; Lustigman, Sara ; Nutman, Thomas B
  • Chủ đề: Proteome ; Symbiosis ; Transcriptome ; Onchocerca Volvulus -- Genetics ; Wolbachia -- Genetics
  • Là 1 phần của: mBio, 23 November 2016, Vol.7(6)
  • Mô tả: Onchocerciasis (river blindness) is a neglected tropical disease that has been successfully targeted by mass drug treatment programs in the Americas and small parts of Africa. Achieving the long-term goal of elimination of onchocerciasis, however, requires additional tools, including drugs, vaccines, and biomarkers of infection. Here, we describe the transcriptome and proteome profiles of the major vector and the human host stages (L1, L2, L3, molting L3, L4, adult male, and adult female) of Onchocerca volvulus along with the proteome of each parasitic stage and of its Wolbachia endosymbiont (wOv). In so doing, we have identified stage-specific pathways important to the parasite's adaptation to its human host during its early development. Further, we generated a protein array that, when screened with well-characterized human samples, identified novel diagnostic biomarkers of O. volvulus infection and new potential vaccine candidates. This immunomic approach not only demonstrates the power of this postgenomic discovery platform but also provides additional tools for onchocerciasis control programs. The global onchocerciasis (river blindness) elimination program will have to rely on the development of new tools (drugs, vaccines, biomarkers) to achieve its goals by 2025. As an adjunct to the completed genomic sequencing of O. volvulus, we used a comprehensive proteomic and transcriptomic profiling strategy to gain a comprehensive understanding of both the vector-derived and human host-derived parasite stages. In so doing, we have identified proteins and pathways that enable novel drug targeting studies and the discovery of novel vaccine candidates, as well as useful biomarkers of active infection.
  • Ngôn ngữ: English
  • Số nhận dạng: E-ISSN: 2150-7511 ; PMID: 27881553 Version:1 ; DOI: 10.1128/mBio.02028-16

Đang tìm Cơ sở dữ liệu bên ngoài...