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Multiple populations of deleted mitochondrial DNA detected by a novel gene amplification method

Sato, W ; Tanaka, M ; Ohno, K ; Yamamoto, T ; Takada, G ; Ozawa, T

Biochemical and biophysical research communications, 31 July 1989, Vol.162(2), pp.664-72 [Tạp chí có phản biện]

ISSN: 0006-291X ; PMID: 2757637 Version:1

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  • Nhan đề:
    Multiple populations of deleted mitochondrial DNA detected by a novel gene amplification method
  • Tác giả: Sato, W ; Tanaka, M ; Ohno, K ; Yamamoto, T ; Takada, G ; Ozawa, T
  • Chủ đề: Chromosome Deletion ; Gene Amplification ; DNA, Mitochondrial -- Analysis ; Muscles -- Analysis ; Ophthalmoplegia -- Genetics
  • Là 1 phần của: Biochemical and biophysical research communications, 31 July 1989, Vol.162(2), pp.664-72
  • Mô tả: A gene amplification method for detecting small populations of deleted mitochondrial DNA was used in analysis of skeletal muscle from a patient with ocular myopathy. Multiple populations of differently deleted mtDNA were detected in the patient muscle. The presence of deleted mtDNAs was further confirmed by comparison of the shift in the sizes of the amplified fragments with the shift in the positions of the primers used for the amplification, (the primer shift PCR method). Other methods, namely Southern blotting, enzymic activity measurement, and Western blotting, were inefficient at detecting the mitochondrial abnormality. These findings suggest that the primer shift PCR method could be valuable for accurate diagnosis of ocular myopathy associated with mtDNA deletion.
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 0006-291X ; PMID: 2757637 Version:1

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