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Decreased frequencies of circulating follicular helper T cell counterparts and plasmablasts in ankylosing spondylitis patients Naïve for TNF blockers

Bautista-Caro, María-Belén ; Arroyo-Villa, Irene ; Castillo-Gallego, Concepción ; De Miguel, Eugenio ; Peiteado, Diana ; Plasencia-Rodríguez, Chamaida ; Villalba, Alejandro ; Sánchez-Mateos, Paloma ; Puig-Kröger, Amaya ; Martín-Mola, Emilio ; Miranda-Carús, María-Eugenia

PloS one, 2014, Vol.9(9), pp.e107086 [Tạp chí có phản biện]

E-ISSN: 1932-6203 ; PMID: 25203742 Version:1 ; DOI: 10.1371/journal.pone.0107086

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  • Nhan đề:
    Decreased frequencies of circulating follicular helper T cell counterparts and plasmablasts in ankylosing spondylitis patients Naïve for TNF blockers
  • Tác giả: Bautista-Caro, María-Belén ; Arroyo-Villa, Irene ; Castillo-Gallego, Concepción ; De Miguel, Eugenio ; Peiteado, Diana ; Plasencia-Rodríguez, Chamaida ; Villalba, Alejandro ; Sánchez-Mateos, Paloma ; Puig-Kröger, Amaya ; Martín-Mola, Emilio ; Miranda-Carús, María-Eugenia
  • Chủ đề: Plasma Cells -- Immunology ; Spondylitis, Ankylosing -- Immunology ; T-Lymphocytes, Helper-Inducer -- Immunology
  • Là 1 phần của: PloS one, 2014, Vol.9(9), pp.e107086
  • Mô tả: Follicular helper T cells (Tfh), localized in lymphoid organs, promote B cell differentiation and function. Circulating CD4 T cells expressing CXCR5, ICOS and/or PD-1 are counterparts of Tfh. Three subpopulations of circulating CD4+CXCR5+ cells have been described: CXCR3+CCR6- (Tfh-Th1), CXCR3-CCR6+ (Tfh-Th17), and CXCR3-CCR6- (Tfh-Th2). Only Tfh-Th17 and Tfh-Th2 function as B cell helpers. Our objective was to study the frequencies of circulating Tfh (cTfh), cTfh subsets and plasmablasts (CD19+CD20-CD27+CD38high cells), and the function of cTfh cells, in patients with Ankylosing Spondylitis (AS). To this end, peripheral blood was drawn from healthy controls (HC) (n = 50), AS patients naïve for TNF blockers (AS/nb) (n = 25) and AS patients treated with TNF blockers (AS/b) (n = 25). The frequencies of cTfh and plasmablasts were determined by flow cytometry. Cocultures of magnetically sorted CD4+CXCR5+ T cells with autologous CD19+CD27- naïve B cells were established from 3 AS/nb patients and 3 HC, and concentrations of IgG, A and M were measured in supernatants. We obseved that AS/nb but not AS/b patients, demonstrated decreased frequencies of circulating CD4+CXCR5+ICOS+PD-1+ cells and plasmablasts, together with a decreased (Tfh-Th17+Tfh-Th2)/Tfh-Th1 ratio. The amounts of IgG and IgA produced in cocultures of CD4+CXCR5+ T cells with CD19+CD27- B cells of AS/nb patients were significantly lower than observed in cocultures established from HC. In summary, AS/nb but not AS/b patients, demonstrate a decreased frequency of cTfh and plasmablasts, and an underrepresentation of cTfh subsets bearing a B helper phenotype. In addition, peripheral blood CD4+CXCR5+ T cells of AS/nb patients showed a decreased capacity to help B cells ex vivo.
  • Ngôn ngữ: English
  • Số nhận dạng: E-ISSN: 1932-6203 ; PMID: 25203742 Version:1 ; DOI: 10.1371/journal.pone.0107086

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