skip to main content
Ngôn ngữ:
Giới hạn tìm kiếm: Giới hạn tìm kiếm: Dạng tài nguyên Hiển thị kết quả với: Hiển thị kết quả với: Chỉ mục

From confluent human iPS cells to self-forming neural retina and retinal pigmented epithelium

Reichman, Sacha ; Terray, Angélique ; Slembrouck, Amélie ; Nanteau, Céline ; Orieux, Gaël ; Habeler, Walter ; Nandrot, Emeline F ; Sahel, José-Alain ; Monville, Christelle ; Goureau, Olivier

Proceedings of the National Academy of Sciences of the United States of America, 10 June 2014, Vol.111(23), pp.8518-23 [Tạp chí có phản biện]

E-ISSN: 1091-6490 ; PMID: 24912154 Version:1 ; DOI: 10.1073/pnas.1324212111

Toàn văn sẵn có

Phiên bản sẵn có
Trích dẫn Trích dẫn bởi
  • Nhan đề:
    From confluent human iPS cells to self-forming neural retina and retinal pigmented epithelium
  • Tác giả: Reichman, Sacha ; Terray, Angélique ; Slembrouck, Amélie ; Nanteau, Céline ; Orieux, Gaël ; Habeler, Walter ; Nandrot, Emeline F ; Sahel, José-Alain ; Monville, Christelle ; Goureau, Olivier
  • Chủ đề: Cones ; Retinal Ganglion Cells ; Rods ; Cell Differentiation ; Induced Pluripotent Stem Cells -- Cytology ; Retinal Neurons -- Cytology ; Retinal Pigment Epithelium -- Cytology
  • Là 1 phần của: Proceedings of the National Academy of Sciences of the United States of America, 10 June 2014, Vol.111(23), pp.8518-23
  • Mô tả: Progress in retinal-cell therapy derived from human pluripotent stem cells currently faces technical challenges that require the development of easy and standardized protocols. Here, we developed a simple retinal differentiation method, based on confluent human induced pluripotent stem cells (hiPSC), bypassing embryoid body formation and the use of exogenous molecules, coating, or Matrigel. In 2 wk, we generated both retinal pigmented epithelial cells and self-forming neural retina (NR)-like structures containing retinal progenitor cells (RPCs). We report sequential differentiation from RPCs to the seven neuroretinal cell types in maturated NR-like structures as floating cultures, thereby revealing the multipotency of RPCs generated from integration-free hiPSCs. Furthermore, Notch pathway inhibition boosted the generation of photoreceptor precursor cells, crucial in establishing cell therapy strategies. This innovative process proposed here provides a readily efficient and scalable approach to produce retinal cells for regenerative medicine and for drug-screening purposes, as well as an in vitro model of human retinal development and disease.
  • Ngôn ngữ: English
  • Số nhận dạng: E-ISSN: 1091-6490 ; PMID: 24912154 Version:1 ; DOI: 10.1073/pnas.1324212111

Đang tìm Cơ sở dữ liệu bên ngoài...