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EPR Analysis of Imidazole Binding to Methanosarcina acetivorans Protoglobin.(Report)

Van Doorslaer, Sabine ; van Den Bosch, Martje ; Tilleman, Lesley ; Dewilde, Sylvia

Applied Magnetic Resonance, 2015, Vol.46(4), p.421(13) [Tạp chí có phản biện]

ISSN: 0937-9347

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  • Nhan đề:
    EPR Analysis of Imidazole Binding to Methanosarcina acetivorans Protoglobin.(Report)
  • Tác giả: Van Doorslaer, Sabine ; van Den Bosch, Martje ; Tilleman, Lesley ; Dewilde, Sylvia
  • Chủ đề: Myoglobin ; Histidine ; Proteins ; Iron Compounds
  • Là 1 phần của: Applied Magnetic Resonance, 2015, Vol.46(4), p.421(13)
  • Mô tả: Byline: Sabine Van Doorslaer (1), Martje van den Bosch (1), Lesley Tilleman (2), Sylvia Dewilde (2) Abstract: Protoglobins form an unusual class of globin proteins with surprising structural features, such as a highly ruffled heme. Electron paramagnetic resonance spectra of the frozen solution of the ferric imidazole complex of Methanosarcina acetivorans protoglobin reveal the existence of two low-spin ferric heme complexes. Hyperfine sublevel correlation spectroscopy was used to study the imidazole complex of this protoglobin as well as the imidazole complex of horse heart myoglobin for comparison. The spin Hamiltonian values obtained for the dominant contribution of the imidazole-ligated protoglobin agree with a heme conformation in which the plane of the exogenous imidazole is turned more than 30[degrees] versus the imidazole plane of the proximal histidine ligand. In turn, the electron paramagnetic resonance data of the minority complex indicate a second conformer, in which the two ligand planes are aligned almost parallel to each other with a lengthened iron--nitrogen bond for the exogenous imidazole ligand. Author Affiliation: (1) BIMEF Laboratory, Department of Physics, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium (2) PPES Laboratory, Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium Article History: Registration Date: 20/12/2014 Received Date: 07/10/2014 Online Date: 09/01/2015 Article note: This paper is in honor of Giovanni Giacometti on the occasion of his 85th birthday. Electronic supplementary material The online version of this article (doi: 10.1007/s00723-014-0638-z) contains supplementary material, which is available to authorized users.
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 0937-9347

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