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Superresolution Imaging of Dynamic MreB Filaments in B. subtilis -- A Multiple-Motor-Driven Transport?

Olshausen, Philipp V. ; Soufo, Herve Joel Defeu ; Wicker, Kai ; Heintzmann, Rainer ; Graumann, Peter L. ; Rohrbach, Alexander

Biophysical Journal, Sept 3, 2013, Vol.105(5), p.1171(11) [Tạp chí có phản biện]

ISSN: 0006-3495

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  • Nhan đề:
    Superresolution Imaging of Dynamic MreB Filaments in B. subtilis -- A Multiple-Motor-Driven Transport?
  • Tác giả: Olshausen, Philipp V. ; Soufo, Herve Joel Defeu ; Wicker, Kai ; Heintzmann, Rainer ; Graumann, Peter L. ; Rohrbach, Alexander
  • Chủ đề: Cytoskeletal Proteins
  • Là 1 phần của: Biophysical Journal, Sept 3, 2013, Vol.105(5), p.1171(11)
  • Mô tả: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.bpj.2013.07.038 Byline: Philipp v. Olshausen, Herve Joel Defeu Soufo, Kai Wicker, Rainer Heintzmann, Peter L. Graumann, Alexander Rohrbach Abstract: The cytoskeletal protein MreB is an essential component of the bacterial cell-shape generation system. Using a superresolution variant of total internal reflection microscopy with structured illumination, as well as three-dimensional stacks of deconvolved epifluorescence microscopy, we found that inside living Bacillus subtilis cells, MreB forms filamentous structures of variable lengths, typically not longer than 1 [mu]m. These filaments move along their orientation and mainly perpendicular to the long bacterial axis, revealing a maximal velocity at an intermediate length and a decreasing velocity with increasing filament length. Filaments move along straight trajectories but can reverse or alter their direction of propagation. Based on our measurements, we provide a mechanistic model that is consistent with all observations. In this model, MreB filaments mechanically couple several motors that putatively synthesize the cell wall, whereas the filaments' traces mirror the trajectories of the motors. On the basis of our mechanistic model, we developed a mathematical model that can explain the nonlinear velocity length dependence. We deduce that the coupling of cell wall synthesis motors determines the MreB filament transport velocity, and the filament mechanically controls a concerted synthesis of parallel peptidoglycan strands to improve cell wall stability. Author Affiliation: (a ) Laboratory for Bio- and Nano-Photonics, Department of Microsystems Engineering-IMTEK, University of Freiburg, Freiburg, Germany (a ) BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany (As.) Department of Microbiology, Faculty of Biology, University of Freiburg, Freiburg, Germany (A[paragraph]) Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich Schiller University Jena, Jena, Germany (||) Institute of Photonic Technology, Jena, Germany (a a ) King's College London, Randall Division, London, UK (a a ) SYNMIKRO, LOEWE Centre for Synthetic Microbiology, University of Marburg, Marburg, Germany (As.As.) Faculty of Chemistry, University of Marburg, Marburg, Germany Article History: Received 24 January 2013; Accepted 26 July 2013 Article Note: (miscellaneous) Editor: David Odde.
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 0006-3495

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