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Improvement of (R)-1,3-butanediol production by engineered Escherichia coli.(Report)

Kataoka, Naoya ; Vangnai, Alisa S. ; Tajima, Takahisa ; Nakashimada, Yutaka ; Kato, Junichi

Journal of Bioscience and Bioengineering, May, 2013, Vol.115(5), p.475(6) [Tạp chí có phản biện]

ISSN: 1389-1723

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  • Nhan đề:
    Improvement of (R)-1,3-butanediol production by engineered Escherichia coli.(Report)
  • Tác giả: Kataoka, Naoya ; Vangnai, Alisa S. ; Tajima, Takahisa ; Nakashimada, Yutaka ; Kato, Junichi
  • Chủ đề: Glucose Metabolism ; Genetic Engineering ; Butanediol ; Hazardous Waste Management ; Genetically Modified Organisms ; Glucose ; Escherichia Coli
  • Là 1 phần của: Journal of Bioscience and Bioengineering, May, 2013, Vol.115(5), p.475(6)
  • Mô tả: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.jbiosc.2012.11.025 Byline: Naoya Kataoka (1), Alisa S. Vangnai (2)(3), Takahisa Tajima (1), Yutaka Nakashimada (1), Junichi Kato (1) Abstract: (R)-1,3-Butanediol is a valuable chemical extensively used as a key intermediate for the synthesis of pharmaceuticals and several industrial compounds. Despite its high demand, the production has been restricted from multi-step chemical production, petrochemical substrate requirement and a non-existence natural synthesis pathway from renewable biomass. In this study, an artificial synthesis route was genetically engineered in Escherichia coli MG1655 lacI.sup.q to produce 1,3-butanediol from glucose. The selection of heterologous genes from several organisms and activity level of their corresponding gene products were demonstrated to be the key element for the product formation. Improvement of fermentation aeration and the application of fed-batch system significantly enhanced 1,3-butanediol production. Under the optimized conditions, 1,3-butanediol was produced up to 9.05 g/l (100.4 mM) with 98.5 [+ or -] 0.2% enantiomeric excess (% ee) of (R)-1,3-butanediol. This is the highest yield of 1,3-butanediol produced from glucose with the highest optical purity by the recombinant strain reported thus far. Author Affiliation: (1) Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Hiroshima 739-8530, Japan (2) Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand (3) Center of Excellence for Environmental and Hazardous Waste Management (EHWM), Chulalongkorn University, Bangkok 10330, Thailand Article History: Received 30 October 2012; Accepted 30 November 2012
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 1389-1723

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