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The small protein MbiA interacts with MreB and modulates cell shape in Caulobacter crescentus.(Report)

Yakhnina, Anastasiya A. ; Gitai, Zemer

Molecular Microbiology, Sept, 2012, Vol.85, p.1090(15) [Tạp chí có phản biện]

ISSN: 0950-382X

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  • Nhan đề:
    The small protein MbiA interacts with MreB and modulates cell shape in Caulobacter crescentus.(Report)
  • Tác giả: Yakhnina, Anastasiya A. ; Gitai, Zemer
  • Chủ đề: Cytoskeletal Proteins ; Muscle Proteins
  • Là 1 phần của: Molecular Microbiology, Sept, 2012, Vol.85, p.1090(15)
  • Mô tả: To purchase or authenticate to the full-text of this article, please visit this link: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2012.08159.x/abstract Byline: Anastasiya A. Yakhnina(1), Zemer Gitai(1) Summary In Caulobacter crescentus, the actin homologue MreB is critical for cell shape maintenance. Despite the central importance of MreB for cell morphology and viability, very little is known about MreB-interacting factors. Here, we use an overexpression approach to identify a novel MreB interactor, MbiA. MbiA interacts with MreB in both biochemical and genetic assays, colocalizes with MreB throughout the cell cycle, and relies on MreB for its localization. MbiA overexpression mimics the loss of MreB function, severely perturbing cell morphology, inhibiting growth and inducing cell lysis. Additionally, mbiA deletion shows a synthetic growth phenotype with a hypomorphic allele of the MreB interactor RodZ, suggesting that these two MreB-interacting proteins either have partially redundant functions or participate in the same functional complex. Our work thus establishes MbiA as a novel cell shape regulator that appears to function through regulating MreB, and opens avenues for discovery of more MreB-regulating factors by showing that overexpression screens are a valuable tool for uncovering potentially redundant cell shape effectors. Author Affiliation: (1)Department of Molecular Biology, Princeton University, Lewis Thomas Laboratory, Princeton, NJ O8544, USA. Correspondence: (*) E-mail zgitai@princeton.edu; Tel. (+1) 609 258 9420; Fax (+1) 609 258 6175. Accepted 27 June, 2012. Supporting information: Additional Supporting Information may be found in the online version of this article
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 0950-382X

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