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The Era GTPase recognizes the GAUCACCUCC sequence and binds helix 45 near the 3' end of 16S rRNA.(BIOCHEMISTRY)(Author abstract)(Report)

Tu, Chao ; Zhou, Xiaomei ; Tarasov, Sergey G. ; Tropea, Joseph E. ; Austin, Brian P. ; Waugh, David S. ; Court, Donald L. ; Ji, Xinhua

Proceedings of the National Academy of Sciences of the United States, June 21, 2011, Vol.108(25), p.10156(6) [Tạp chí có phản biện]

ISSN: 0027-8424

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  • Nhan đề:
    The Era GTPase recognizes the GAUCACCUCC sequence and binds helix 45 near the 3' end of 16S rRNA.(BIOCHEMISTRY)(Author abstract)(Report)
  • Tác giả: Tu, Chao ; Zhou, Xiaomei ; Tarasov, Sergey G. ; Tropea, Joseph E. ; Austin, Brian P. ; Waugh, David S. ; Court, Donald L. ; Ji, Xinhua
  • Chủ đề: Nucleotides -- Properties ; Gtpases -- Properties ; Protein-protein Interactions -- Research
  • Là 1 phần của: Proceedings of the National Academy of Sciences of the United States, June 21, 2011, Vol.108(25), p.10156(6)
  • Mô tả: Era, composed of a GTPase domain and a K homology domain, is essential for bacterial cell viability. It is required for the maturation of 16S rRNA and assembly of the 30S ribosomal subunit. We showed previously that the protein recognizes nine nucleotides ([sub.1531][AUCACCUCC.sub.1539]) near the 3' end of 16S rRNA, and that this recognition stimulates GTP-hydrolyzing activity of Era. In all three kingdoms of life, the [sub.1530][GAUCA.sub.1534] sequence and helix 45 (h45) (nucleotides 1506-1529) are highly conserved. It has been shown that the [sub.1530][GA.sub.1531] to [sub.1530][AG.sub.1531] double mutation severely affects the viability of bacteria. However, whether Era interacts with G1530 and/or h45 and whether such interactions (if any) contribute to the stimulation of Era's GTPase activity were not known. Here, we report two RNA structures that contain nucleotides 1506-1542 (RNA301), one in complex with Era and GDPNP (GNP), a nonhydrolysable GTP-analogue, and the other in complex with Era, GNP, and the KsgA methyltransferase. The structures show that Era recognizes 10 nucleotides, including G1530, and that Era also binds h45. Moreover, GTPase assay experiments show that G1530 does not stimulate Era's GTPase activity. Rather, A1531 and A1534 are most important for stimulation and h45 further contributes to the stimulation. Although G1530 does not contribute to the intrinsic GTPase activity of Era, its interaction with Era is important for binding and is essential for the protein to function, leading to the discovery of a new cold-sensitive phenotype of Era. ribosome biogenesis | cell growth regulation doi/ 10.1073/pnas.1017679108
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 0027-8424

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