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Structural basis for piRNA 2'-O-methylated 3'-end recognition by Piwi PAZ (Piwi/Argonaute/Zwille) domains.(INAUGURAL ARTICLES)(Author abstract)(Report)

Tian, Yuan ; Simanshu, Dhirendra K. ; Ma, Jin - Biao ; Patel, Dinshaw J.

Proceedings of the National Academy of Sciences of the United States, Jan 18, 2011, Vol.108(3), p.903(8) [Tạp chí có phản biện]

ISSN: 0027-8424

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  • Nhan đề:
    Structural basis for piRNA 2'-O-methylated 3'-end recognition by Piwi PAZ (Piwi/Argonaute/Zwille) domains.(INAUGURAL ARTICLES)(Author abstract)(Report)
  • Tác giả: Tian, Yuan ; Simanshu, Dhirendra K. ; Ma, Jin - Biao ; Patel, Dinshaw J.
  • Chủ đề: Proteins -- Properties ; Rna -- Research
  • Là 1 phần của: Proceedings of the National Academy of Sciences of the United States, Jan 18, 2011, Vol.108(3), p.903(8)
  • Mô tả: Argonaute and Piwi proteins are key players in the RNA silencing pathway, with the former interacting with micro-RNAs (miRNAs) and siRNAs, whereas the latter targets piwi-interacting RNAs (piRNAs) that are 2'-O-methylated (2r-OCH3) at their 3' ends. Germline-specific piRNAs and Piwi proteins play a critical role in genome defense against transposable elements, thereby protecting the genome against transposon-induced defects in gametogenesis and fertility. Humans contain four Piwi family proteins designated Hiwil, Hiwi2, Hiwi3, and Hill. We report on the structures of Hili-PAZ (Piwi/Argonaute/Zwille) domain in the free state and Hiwi1 PAZ domain bound to self-complementary 14-met RNAs (12-bp + 2-nt overhang) containing 2'-OC[H.sub.3] and 2'-OH at their 3' ends. These structures explain the molecular basis underlying accommodation of the 2-OCH3 group within a preformed Hiwi1 PAZ domain binding pocket, whose hydrophobic characteristics account for the preferential binding of 2'-OC[H.sub.3] over 2'-OH 3' ends. These results contrast with the more restricted binding pocket for the human Ago1 PAZ domain, which exhibits a reverse order, with preferential binding of 2'-OH over 2t-OCH3 3' ends. doi/ 10.1073/pnas.1017762108
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 0027-8424

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