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Oligosaccharyltransferase directly binds to ribosome at a location near the translocon-binding site.(BIOCHEMISTRY)(Author abstract)(Report)

Harada, Yoichiro ; Li, Hua ; Li, Huilin ; Lennarz, William J.

Proceedings of the National Academy of Sciences of the United States, April 28, 2009, Vol.106(17), p.6945(5) [Tạp chí có phản biện]

ISSN: 0027-8424

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  • Nhan đề:
    Oligosaccharyltransferase directly binds to ribosome at a location near the translocon-binding site.(BIOCHEMISTRY)(Author abstract)(Report)
  • Tác giả: Harada, Yoichiro ; Li, Hua ; Li, Huilin ; Lennarz, William J.
  • Chủ đề: Oligosaccharides -- Properties ; Transferases -- Properties ; Ribosomes -- Properties ; Protein Binding -- Research
  • Là 1 phần của: Proceedings of the National Academy of Sciences of the United States, April 28, 2009, Vol.106(17), p.6945(5)
  • Mô tả: Oligosaccharyltransferase (OT) transfers high mannose-type glycans to the nascent polypeptides that are translated by the membrane-bound ribosome and translocated into the lumen of the endoplasmic reticulum through the Sec61 translocon complex. In this article, we show that purified ribosomes and OT can form a binary complex with a stoichiometry of [approximately equal to] 1 to 1 in the presence of detergent. We present evidence that OT may bind to the large ribosomal subunit near the site where nascent polypeptides exit. We further show that OT and the Sec61 complex can simultaneously bind to ribosomes in vitro. Based on existing data and our findings, we propose that cotranslational translocation and N-glycosylation of nascent polypeptides are mediated by a ternary supramolecular complex consisting of OT, the Sec61 complex, and ribosomes. electron microscopy | glycoprotein biosynthesis | multicomponent complexes
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 0027-8424

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