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Development of a novel system for producing ajmalicine and serpentine using direct culture of leaves in Catharanthus roseus intact plant.(Report)

Iwase, Akira ; Aoyagi, Hideki ; Ohme - Takagi, Masaru ; Tanaka, Hideo

Journal of Bioscience and Bioengineering, March, 2005, Vol.99(3), p.208(8) [Tạp chí có phản biện]

ISSN: 1389-1723

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  • Nhan đề:
    Development of a novel system for producing ajmalicine and serpentine using direct culture of leaves in Catharanthus roseus intact plant.(Report)
  • Tác giả: Iwase, Akira ; Aoyagi, Hideki ; Ohme - Takagi, Masaru ; Tanaka, Hideo
  • Chủ đề: Glucose -- Analysis ; Polyketides -- Analysis ; Herbal Medicine -- Analysis ; Gene Expression -- Analysis ; Environmental Sciences -- Analysis ; Enzymes -- Analysis ; Serpentine -- Analysis ; Alkaloids -- Analysis ; Plant Metabolites -- Analysis
  • Là 1 phần của: Journal of Bioscience and Bioengineering, March, 2005, Vol.99(3), p.208(8)
  • Mô tả: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1263/jbb.99.208 Byline: Akira Iwase (a), Hideki Aoyagi (a), Masaru Ohme-Takagi (b), Hideo Tanaka (a) Keywords: Catharanthus roseus; cell culture; terpenoid indole alkaloid; secondary metabolite; biosynthesis Abstract: Due to problems of production instability, the production of plant secondary metabolites using dedifferentiated cells (callus) is not always feasible on an industrial scale. To propose a new methodology, which does not use dedifferentiated cells, a novel system for producing useful secondary metabolites using the direct culture of intact plant leaves was developed. Catharanthus roseus was used as a model medicinal plant to produce terpenoid indole alkaloids (TIAs) by suspension culture of the leaves in the phytohormone-free MS liquid medium. Adjustment of the osmotic pressure (993 kPa at 25[degrees]C) in the medium, light irradiation (60 [mu]mol m.sup.-2 s.sup.-1) and addition of glucose (10 g/l) were effective to promote the production of TIAs such as ajmalicine (Aj) and serpentine (Sp). On the basis of semi-quantitative RT-PCR analyses, it was revealed that the culture conditions promoted gene expression of enzymes in the TIA pathway in the cultured leaves. By feeding glucose (10 g/l) on day 10 of the culture period, Aj was produced at a concentration of about 18 mg/l and Sp was produced at a concentration about 11-fold that of the control. These results represent the first step in the development of a novel production system for plant secondary metabolites. Author Affiliation: (a) Life Science and Bioengineering, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan (b) Gene Function Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Central 4, Tsukuba, Ibaraki 305-8562, Japan Article History: Received 10 December 2004; Accepted 11 January 2005
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 1389-1723

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