skip to main content
Ngôn ngữ:
Giới hạn tìm kiếm: Giới hạn tìm kiếm: Dạng tài nguyên Hiển thị kết quả với: Hiển thị kết quả với: Chỉ mục

SAMPLEX: Automatic mapping of perturbed and unperturbed regions of proteins and complexes

Loth Karine ; Krzeminski Mickaël ; Boelens Rolf ; Bonvin Alexandre Mjj

BMC Bioinformatics, 01 January 2010, Vol.11(1), p.51 [Tạp chí có phản biện]

ISSN: 1471-2105 ; E-ISSN: 1471-2105 ; DOI: 10.1186/1471-2105-11-51

Toàn văn sẵn có

Trích dẫn Trích dẫn bởi
  • Nhan đề:
    SAMPLEX: Automatic mapping of perturbed and unperturbed regions of proteins and complexes
  • Tác giả: Loth Karine ; Krzeminski Mickaël ; Boelens Rolf ; Bonvin Alexandre Mjj
  • Chủ đề: Biology
  • Là 1 phần của: BMC Bioinformatics, 01 January 2010, Vol.11(1), p.51
  • Mô tả: Abstract Background The activity of proteins within the cell is characterized by their motions, flexibility, interactions or even the particularly intriguing case of partially unfolded states. In the last two cases, a part of the protein is affected either by binding or unfolding and the detection of the respective perturbed and unperturbed region(s) is a fundamental part of the structural characterization of these states. This can be achieved by comparing experimental data of the same protein in two different states (bound/unbound, folded/unfolded). For instance, measurements of chemical shift perturbations (CSPs) from NMR 1H-15N HSQC experiments gives an excellent opportunity to discriminate both moieties. Results We describe an innovative, automatic and unbiased method to distinguish perturbed and unperturbed regions in a protein existing in two distinct states (folded/partially unfolded, bound/unbound). The SAMPLEX program takes as input a set of data and the corresponding three-dimensional structure and returns the confidence for each residue to be in a perturbed or unperturbed state. Its performance is demonstrated for different applications including the prediction of disordered regions in partially unfolded proteins and of interacting regions in protein complexes. Conclusions The proposed approach is suitable for partially unfolded states of proteins, local perturbations due to small ligands and protein-protein interfaces. The method is not restricted to NMR data, but is generic and can be applied to a wide variety of information.
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 1471-2105 ; E-ISSN: 1471-2105 ; DOI: 10.1186/1471-2105-11-51

Đang tìm Cơ sở dữ liệu bên ngoài...