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Full-length title: Dramatic HIV DNA degradation associated with spontaneous HIV suppression and disease-free outcome in a young seropositive woman following her infection

Philippe Colson ; Catherine Dhiver ; Catherine Tamalet ; Jeremy Delerce ; Olga O. Glazunova ; Maxime Gaudin ; Anthony Levasseur ; Didier Raoult

Scientific reports, 01 February 2020, Vol.10(1), pp.1-9 [Tạp chí có phản biện]

E-ISSN: 2045-2322 ; DOI: 10.1038/s41598-020-58969-6

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  • Nhan đề:
    Full-length title: Dramatic HIV DNA degradation associated with spontaneous HIV suppression and disease-free outcome in a young seropositive woman following her infection
  • Tác giả: Philippe Colson ; Catherine Dhiver ; Catherine Tamalet ; Jeremy Delerce ; Olga O. Glazunova ; Maxime Gaudin ; Anthony Levasseur ; Didier Raoult
  • Chủ đề: Biology
  • Là 1 phần của: Scientific reports, 01 February 2020, Vol.10(1), pp.1-9
  • Mô tả: Abstract Strategies to cure HIV-infected patients by virus-targeting drugs have failed to date. We identified a HIV-1-seropositive woman who spontaneously suppressed HIV replication and had normal CD4-cell counts, no HIV-disease, no replication-competent virus and no cell HIV DNA detected with a routine assay. We suspected that dramatic HIV DNA degradation occurred post-infection. We performed multiple nested-PCRs followed by Sanger sequencing and applied a multiplex-PCR approach. Furthermore, we implemented a new technique based on two hybridization steps on beads prior to next-generation sequencing that removed human DNA then retrieved integrated HIV sequences with HIV-specific probes. We assembled ≈45% of the HIV genome and further analyzed the G-to-A mutations putatively generated by cellular APOBEC3 enzymes that can change tryptophan codons into stop codons. We found more G-to-A mutations in the HIV DNA from the woman than in that of her transmitting partner. Moreover, 74%...
  • Ngôn ngữ: English
  • Số nhận dạng: E-ISSN: 2045-2322 ; DOI: 10.1038/s41598-020-58969-6

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