skip to main content
Ngôn ngữ:
Giới hạn tìm kiếm: Giới hạn tìm kiếm: Dạng tài nguyên Hiển thị kết quả với: Hiển thị kết quả với: Chỉ mục

Delayed Activation of Spinal Microglia Contributes to the Maintenance of Bone Cancer Pain in Female Wistar Rats via P2X7 Receptor and IL-18

Yang, Y. ; Li, H. ; Li, T.-T. ; Luo, H. ; Gu, X.-Y. ; Lu, N. ; Ji, R.-R. ; Zhang, Y.-Q.

Journal of Neuroscience, 05/20/2015, Vol.35(20), pp.7950-7963 [Tạp chí có phản biện]

ISSN: 0270-6474 ; E-ISSN: 1529-2401 ; DOI: http://dx.doi.org/10.1523/JNEUROSCI.5250-14.2015

Toàn văn sẵn có

Trích dẫn Trích dẫn bởi
  • Nhan đề:
    Delayed Activation of Spinal Microglia Contributes to the Maintenance of Bone Cancer Pain in Female Wistar Rats via P2X7 Receptor and IL-18
  • Tác giả: Yang, Y. ; Li, H. ; Li, T.-T. ; Luo, H. ; Gu, X.-Y. ; Lu, N. ; Ji, R.-R. ; Zhang, Y.-Q.
  • Chủ đề: Anatomy & Physiology
  • Là 1 phần của: Journal of Neuroscience, 05/20/2015, Vol.35(20), pp.7950-7963
  • Mô tả: Accumulating evidence suggests that activation of spinal microglia contributes to the development of inflammatory and neuropathic pain. However, the role of spinal microglia in the maintenance of chronic pain remains controversial. Bone cancer pain shares features of inflammatory and neuropathic pain, but the temporal activation of microglia and astrocytes in this model is not well defined. Here, we report an unconventional role of spinal microglia in the maintenance of advanced-phase bone cancer pain in a female rat model. Bone cancer elicited delayed and persistent microglial activation in the spinal dorsal horn on days 14 and 21, but not on day 7. In contrast, bone cancer induced rapid and persistent astrocytic activation on days 7-21. Spinal inhibition of microglia by minocycline at 14 d effectively reduced bone cancer-induced allodynia and hyperalgesia. However, pretreatment of minocycline in the first week did not affect the development of cancer pain. Bone cancer increased ATP levels in CSF, and upregulated P2X7 receptor, phosphorylated p38, and IL-18 in spinal microglia. Spinal inhibition of P2X7/p-38/IL-18 pathway reduced advanced-phase bone cancer pain and suppressed hyperactivity of spinal wide dynamic range (WDR) neurons. IL-18 induced allodynia and hyperalgesia after intrathecal injection, elicited mechanical hyperactivity of WDR neurons in vivo, and increased the frequency of mEPSCs in spinal lamina IIo nociceptive synapses in spinal cord slices. Together, our findings demonstrate a novel role of microglia in maintaining advanced phase cancer pain in females via producing the proinflammatory cytokine IL-18 to enhance synaptic transmission of spinal cord nociceptive neurons.
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 0270-6474 ; E-ISSN: 1529-2401 ; DOI: http://dx.doi.org/10.1523/JNEUROSCI.5250-14.2015

Đang tìm Cơ sở dữ liệu bên ngoài...