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Biodegradation of high-molecular weight PAHs by Rhodococcus wratislaviensis strain 9: Overexpression of amidohydrolase induced by pyrene and BaP

Subashchandrabose, Suresh R. ; Venkateswarlu, Kadiyala ; Naidu, Ravi ; Megharaj, Mallavarapu

Science of The Total Environment, 02/2019, Vol.651, P1, pp.813-821 [Tạp chí có phản biện]

ISSN: 00489697 ; DOI: http://dx.doi.org/10.1016/j.scitotenv.2018.09.192

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  • Nhan đề:
    Biodegradation of high-molecular weight PAHs by Rhodococcus wratislaviensis strain 9: Overexpression of amidohydrolase induced by pyrene and BaP
  • Tác giả: Subashchandrabose, Suresh R. ; Venkateswarlu, Kadiyala ; Naidu, Ravi ; Megharaj, Mallavarapu
  • Chủ đề: Surface Active Agents – Physiological Aspects ; Biodegradation – Physiological Aspects ; Benzopyrene – Physiological Aspects ; Unsaturated Fatty Acids – Physiological Aspects
  • Là 1 phần của: Science of The Total Environment, 02/2019, Vol.651, P1, pp.813-821
  • Mô tả: Byline: Suresh R. Subashchandrabose (a), Kadiyala Venkateswarlu (b), Ravi Naidu (a), Mallavarapu Megharaj [megh.mallavarapu@newcastle.edu.au] (a,*) Keywords Rhodococcus wratislaviensis strain 9; PAHs biodegradation; Pyrene; BaP; Amidohydrolase overexpresssion Highlights * Rhodococcus wratislaviensis strain 9 degrades phenanthrene, pyrene and BaP. * Strain 9 harbors several major genes implicated in hydrocarbon biodegradation. * Bioavailability is the major limiting factor in bacterial degradation of BaP. * Phenanthrene induces catechol 2,3-dioxygenase and the large subunit of ndo. * Pyrene and BaP induce the overexpression of metallo-dependent amidohydrolase. Abstract A Gram-positive bacterium, Rhodococcus wratislaviensis strain 9, completely degraded 280 [mu]M of phenanthrene, 40% of 50 [mu]M pyrene or 28% of 40 [mu]M benzo[a]pyrene (BaP), each supplemented in M9 medium, within 7 days. PCR screening with gene-specific primers indicated that the strain 9 harbors genes which code for 2,3-dihydroxybiphenyl 1,2-dioxygenase (bphC), 4-nitrophenol 2-monooxygenase component B (npcB) as well as oxygenase component (nphA1), 4-hydroxybenzoate 3-monooxygenase (phbH), extradiol dioxygenase (edo), and naphthalene dioxygenase (ndo), all of which are largely implicated in biodegradation of several aromatic hydrocarbons. An orthogonal design experiment revealed that BaP biodegradation was greatly enhanced by surfactants such as Tween 80, Triton X-100 and linoleic acid, suggesting that bioavailability is the major limiting factor in bacterial metabolism of BaP. Both pyrene and BaP induced the overexpression of amidohydrolase, a metallo-dependent hydrolase, possibly involved in their biodegradation by strain 9. The up-regulation of amidohydrolase gene induced by BaP, in particular, was also confirmed by semi-quantitative RT-PCR. Catechol 2,3-dioxygenase and the large subunit of ndo, but not amidohydrolase, accumulated when the strain 9 was grown on phenanthrene. To our knowledge, this is the first report on overexpression of amidohydrolase and its possible implication in bacterial degradation of high-molecular weight PAHs. Author Affiliation: (a) Global Centre for Environmental Remediation (GCER), Faculty of Science, The University of Newcastle, Callaghan NSW 2308, Australia, and CRC CARE, Newcastle University LPO, PO Box 18, Callaghan, NSW 2308, Australia (b) Formerly Department of Microbiology, Sri Krishnadevaraya University, Anantapur 515055, India * Corresponding author at: Global Centre for Environmental Remediation (GCER), Faculty of Science, The University of Newcastle, ATC Building, University Drive, Callaghan, NSW 2308, Australia. Article History: Received 4 July 2018; Revised 14 September 2018; Accepted 15 September 2018 (miscellaneous) Editor: Jose Julio Ortega-Calvo
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 00489697 ; DOI: http://dx.doi.org/10.1016/j.scitotenv.2018.09.192

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