skip to main content
Ngôn ngữ:
Giới hạn tìm kiếm: Giới hạn tìm kiếm: Dạng tài nguyên Hiển thị kết quả với: Hiển thị kết quả với: Chỉ mục

Molecular cloning, expression and polymorphism of the porcine apolipoprotein A5 gene in a Jinhua × Pietrain F 2 reference population

Zhang, L. F ; Jiang, X. L ; Hua, X. C ; Lu, Y. P ; Xu, N. Y

animal, 2010, Vol.4(4), pp.523-529 [Tạp chí có phản biện]

ISSN: 1751-7311 ; E-ISSN: 1751-732X ; DOI: 10.1017/S175173110999142X

Toàn văn sẵn có

Phiên bản sẵn có
Trích dẫn Trích dẫn bởi
  • Nhan đề:
    Molecular cloning, expression and polymorphism of the porcine apolipoprotein A5 gene in a Jinhua × Pietrain F 2 reference population
  • Tác giả: Zhang, L. F ; Jiang, X. L ; Hua, X. C ; Lu, Y. P ; Xu, N. Y
  • Chủ đề: Breeding And Genetics; Porcine; Gene; Cloning; Expression; Polymorphism
  • Là 1 phần của: animal, 2010, Vol.4(4), pp.523-529
  • Mô tả: As a newly described member of the apolipoprotein gene family, apolipoprotein A5 ( APOA5 ) has been suggested to play a key role in the triglyceride metabolism in both human and mice. The aim of this study was to identify the porcine (Sus scrofa) APOA5 gene, determine its mRNA and its mutations that are associated with lipid accumulation. The porcine APOA5 cDNA was amplified by reverse transcriptase polymerase chain reaction using the information of the mouse or other mammals. It had been determined that the open reading frame of the porcine APOA5 gene consists of 1092 bp, which encodes a predicted protein composed of 363 amino acids with a similarity to bovine (80.43%) and to human (78.47%). The expression analysis indicated that the porcine APOA5 gene was expressed in hypophysis, fat and liver. Twelve single nucleotide polymorphisms (SNPs), including 4 SNPs in the 5′ end, 1 SNP in second intron, 1 SNP in third exon and 6 SNPs in the 3′ end, were identified in the porcine APOA5 gene and genotyped on the Jinhua × Pietrain F 2 reference population, it had revealed that the SNP of C1834T was significantly associated with average backfat thickness and leaf fat weight ( P < 0.01 and P < 0.05, respectively). In conclusion, this study has got basic information of the porcine APOA5 gene and provides evidence that the APOA5 gene could be a potential candidate gene for fat deposition.
  • Số nhận dạng: ISSN: 1751-7311 ; E-ISSN: 1751-732X ; DOI: 10.1017/S175173110999142X

Đang tìm Cơ sở dữ liệu bên ngoài...