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Microfluidic integration for automated targeted proteomic assays

Hughes, Alex J ; Lin, Robert K C ; Peehl, Donna M ; Herr, Amy E

Proceedings of the National Academy of Sciences of the United States of America, 17 April 2012, Vol.109(16), pp.5972-7 [Tạp chí có phản biện]

E-ISSN: 1091-6490 ; PMID: 22474344 Version:1 ; DOI: 10.1073/pnas.1108617109

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  • Nhan đề:
    Microfluidic integration for automated targeted proteomic assays
  • Tác giả: Hughes, Alex J ; Lin, Robert K C ; Peehl, Donna M ; Herr, Amy E
  • Chủ đề: Biomarkers, Tumor -- Analysis ; Microfluidics -- Methods ; Proteome -- Analysis ; Proteomics -- Methods
  • Là 1 phần của: Proceedings of the National Academy of Sciences of the United States of America, 17 April 2012, Vol.109(16), pp.5972-7
  • Mô tả: A dearth of protein isoform-based clinical diagnostics currently hinders advances in personalized medicine. A well-organized protein biomarker validation process that includes facile measurement of protein isoforms would accelerate development of effective protein-based diagnostics. Toward scalable protein isoform analysis, we introduce a microfluidic "single-channel, multistage" immunoblotting strategy. The multistep assay performs all immunoblotting steps: separation, immobilization of resolved proteins, antibody probing of immobilized proteins, and all interim wash steps. Programmable, low-dispersion electrophoretic transport obviates the need for pumps and valves. A three-dimensional bulk photoreactive hydrogel eliminates manual blotting. In addition to simplified operation and interfacing, directed electrophoretic transport through our 3D nanoporous reactive hydrogel yields superior performance over the state-of-the-art in enhanced capture efficiency (on par with membrane electroblotting)...
  • Ngôn ngữ: English
  • Số nhận dạng: E-ISSN: 1091-6490 ; PMID: 22474344 Version:1 ; DOI: 10.1073/pnas.1108617109

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