skip to main content
Ngôn ngữ:
Giới hạn tìm kiếm: Giới hạn tìm kiếm: Dạng tài nguyên Hiển thị kết quả với: Hiển thị kết quả với: Chỉ mục

Cell wall integrity modulates RHO1 activity via the exchange factor ROM2

Bickle, M ; Delley, P A ; Schmidt, A ; Hall, M N

The EMBO journal, 15 April 1998, Vol.17(8), pp.2235-45 [Tạp chí có phản biện]

ISSN: 0261-4189 ; PMID: 9545237 Version:1

Toàn văn sẵn có

Trích dẫn Trích dẫn bởi
  • Nhan đề:
    Cell wall integrity modulates RHO1 activity via the exchange factor ROM2
  • Tác giả: Bickle, M ; Delley, P A ; Schmidt, A ; Hall, M N
  • Chủ đề: Rho Gtp-Binding Proteins ; Gtp Phosphohydrolases -- Metabolism ; Gtp-Binding Proteins -- Metabolism ; Proteins -- Metabolism ; Saccharomyces Cerevisiae -- Metabolism
  • Là 1 phần của: The EMBO journal, 15 April 1998, Vol.17(8), pp.2235-45
  • Mô tả: The essential phosphatidylinositol kinase homologue TOR2 of Saccharomyces cerevisiae controls the actin cytoskeleton by activating a GTPase switch consisting of RHO1 (GTPase), ROM2 (GEF) and SAC7 (GAP). We have identified two mutations, rot1-1 and rot2-1, that suppress the loss of TOR2 and are synthetic-lethal. The wild-type ROT1 and ROT2 genes and a multicopy suppressor, BIG1, were isolated by their ability to rescue the rot1-1 rot2-1 double mutant. ROT2 encodes glucosidase II, and ROT1 and BIG1 encode novel proteins. We present evidence that cell wall defects activate RHO1. First, rot1, rot2, big1, cwh41, gas1 and fks1 mutations all confer cell wall defects and suppress tor2(ts). Second, destabilizing the cell wall by supplementing the growth medium with 0.005% SDS also suppresses a tor2(ts) mutation. Third, disturbing the cell wall with SDS or a rot1, rot2, big1, cwh41, gas1 or fks1 mutation increases GDP/GTP exchange activity toward RHO1. These results suggest that cell wall defects suppress a tor2 mutation by activating RHO1 independently of TOR2, thereby inducing TOR2-independent polarization of the actin cytoskeleton and cell wall synthesis. Activation of RHO1, a subunit of the cell wall synthesis enzyme glucan synthase, by a cell wall alteration would ensure that cell wall synthesis occurs only when and where needed. The mechanism of RHO1 activation by a cell wall alteration is via the exchange factor ROM2 and could be analogous to signalling by integrin receptors in mammalian cells.
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 0261-4189 ; PMID: 9545237 Version:1
Liên kết riêng
Trở lại danh sách kết quả

    DANH MỤC LIÊN HỆ
  Giới thiệu Tìm kiếm    Nhà C1T, Đại học Quốc gia Hà Nội
  Sản phẩm Sử dụng       144 Xuân Thủy, Cầu Giấy, Hà Nội
  Dịch vụ Hỏi đáp    (024) 3754 6545
  Tài liệu mở    lic@vnu.edu.vn

     ©2016 Library and Information Center, Vietnam National University, Hanoi. All Right Reserved.
     ©2016 Library and Information Center, Vietnam National University, Hanoi. All Right Reserved.
  Nhà C1T, Đại học Quốc gia Hà Nội
144 Xuân Thủy, Cầu Giấy, Hà Nội
  (024) 3754 6545    lic@vnu.edu.vn

Đang tìm Cơ sở dữ liệu bên ngoài...