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Fluorescent reporter genes and the analysis of bacterial regulatory networks

De Jong, Hidde ; Geiselmann, Johannes; De Jong, Hidde (Editor)

Hybrid Systems Biology: Proceedings of the Second International Workshop, HSB 2013, and Third International Workshop, HSB 2014, 2015, Vol.7699, pp.1-24 [Tạp chí có phản biện]

DOI: 10.1007/978-3-319-27656-4_2

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  • Nhan đề:
    Fluorescent reporter genes and the analysis of bacterial regulatory networks
  • Tác giả: De Jong, Hidde ; Geiselmann, Johannes
  • De Jong, Hidde (Editor)
  • Chủ đề: Life Sciences ; Quantitative Methods
  • Là 1 phần của: Hybrid Systems Biology: Proceedings of the Second International Workshop, HSB 2013, and Third International Workshop, HSB 2014, 2015, Vol.7699, pp.1-24
  • Mô tả: The understanding of the regulatory networks controllingthe adaptation of bacteria to changes in their environment is criticallydependent on the ability to monitor the dynamics of gene expression.Here, we review the use of fluorescent reporter genes for dynamicallyquantifying promoter activity and other quantities characterizing geneexpression. We discuss critical physical and biological parameters in thedesign, development, and use of fluorescent reporter strains. Moreover,we review measurement models that have been proposed to interpretprimary fluorescence data and inference methods for estimating geneexpression profiles from these data. As an illustration of the use of fluorescentreporter strains for analyzing bacterial regulatory networks, weconsider two applications in the model bacterium Escherichia coli insome detail: the joint control of gene expression by global physiologicaleffects and specific regulatory interactions, and the importance of proteinstability for the...
  • Ngôn ngữ: English
  • Số nhận dạng: DOI: 10.1007/978-3-319-27656-4_2

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