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EPR analysis of cyanide complexes of wild-type human neuroglobin and mutants in comparison to horse heart myoglobin

Van Doorslaer, Sabine ; Trandafir, Florin ; Harmer, Jeffrey R. ; Moens, Luc ; Dewilde, Sylvia

Biophysical Chemistry, June, 2014, Vol.190-191, p.8(9) [Tạp chí có phản biện]

ISSN: 0301-4622

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  • Nhan đề:
    EPR analysis of cyanide complexes of wild-type human neuroglobin and mutants in comparison to horse heart myoglobin
  • Tác giả: Van Doorslaer, Sabine ; Trandafir, Florin ; Harmer, Jeffrey R. ; Moens, Luc ; Dewilde, Sylvia
  • Chủ đề: Myoglobin -- Analysis ; Cyanides -- Analysis ; Proteins -- Analysis
  • Là 1 phần của: Biophysical Chemistry, June, 2014, Vol.190-191, p.8(9)
  • Mô tả: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.bpc.2014.03.007 Byline: Sabine Van Doorslaer, Florin Trandafir, Jeffrey R. Harmer, Luc Moens, Sylvia Dewilde Abstract: Electron paramagnetic resonance (EPR) data reveal large differences between the ferric (.sup.13C-)cyanide complexes of wild-type human neuroglobin (NGB) and its H64Q and F28L point mutants and the cyanide complexes of mammalian myo- and haemoglobin. The point mutations, which involve residues comprising the distal haem pocket in NGB, induce smaller, but still significant changes, related to changes in the stabilization of the cyanide ligand. Furthermore, for the first time, the full.sup.13C hyperfine tensor of the cyanide carbon of cyanide-ligated horse heart myoglobin (hhMb) was determined using Davies ENDOR (electron nuclear double resonance). Disagreement of these experimental data with earlier predictions based on.sup.13C NMR data and a theoretical model reveal significant flaws in the model assumptions. The same ENDOR procedure allowed also partial determination of the corresponding.sup.13C hyperfine tensor of cyanide-ligated NGB and H64QNGB. These.sup.13C parameters differ significantly from those of cyanide-ligated hhMb and challenge our current theoretical understanding of how the haem environment influences the magnetic parameters obtained by EPR and NMR in cyanide-ligated haem proteins. Article History: Received 20 February 2014; Revised 21 March 2014; Accepted 28 March 2014
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 0301-4622

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