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Kinetics of lactose conversion to galacto-oligosaccharides by [beta]-galactosidase immobilized on PVDF membrane

Palai, Tapas ; Bhattacharya, Prashant K.

Journal of Bioscience and Bioengineering, June, 2013, Vol.115(6), p.668(6) [Tạp chí có phản biện]

ISSN: 1389-1723

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  • Nhan đề:
    Kinetics of lactose conversion to galacto-oligosaccharides by [beta]-galactosidase immobilized on PVDF membrane
  • Tác giả: Palai, Tapas ; Bhattacharya, Prashant K.
  • Chủ đề: Galactose -- Analysis ; Glucose Metabolism -- Analysis ; Enzymes -- Analysis ; Enzymology -- Analysis ; Restaurants -- Analysis ; Glucose -- Analysis ; Polyvinylidene Fluoride -- Analysis ; Lactose -- Analysis
  • Là 1 phần của: Journal of Bioscience and Bioengineering, June, 2013, Vol.115(6), p.668(6)
  • Mô tả: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.jbiosc.2012.12.014 Byline: Tapas Palai, Prashant K. Bhattacharya Abstract: Experimental studies were made for immobilization of enzymes on microporous polyvinylidene fluoride (PVDF) membrane in order to carry out enzymatic reaction of lactose into galacto-oligosaccharides using [beta]-galactosidase. The present work, however, is the first part in the direction of enzymatic membrane reactor studies for carrying out reaction followed by membrane based separation to purify galacto-oligosaccharides out of reaction mixture. The middle of the three compartment cell, separated by two immobilized (enzyme) membranes, was utilized to feed lactose solution; whereas, adjacent compartments were filled with distilled water. The reacted mixture solution was analyzed for tri-, tetra- and penta-forms of GOS. The formation of product GOS strongly depended on varying amounts of initial lactose concentration (ILC). Total GOS formation increased from 7% to 28% for ILC from 50 to 200 g/L. However, tri-saccharide was the major (67%) in comparison to tetra (27%) and penta (6%) forms of GOS. Further, based on Michaelis-Menten kinetics, a six-step-eleven-parameter model was developed. The model incorporated enzyme inhibition and formation of glucose and galactose separately. Simulated results from developed model matched exceeding well with experimental results. Author Affiliation: Department of Chemical Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, India Article History: Received 28 August 2012; Accepted 18 December 2012
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 1389-1723

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